axio observer inverted epifluorescence microscope  (Carl Zeiss)

Journal: bioRxiv

Article Title: Extracellular vesicles from triple negative breast cancer cells disrupt the blood–brain barrier via miR-146a-5p- and TGF-β1-mediated downregulation of endothelial Paqr5

doi: 10.64898/2026.01.26.701753

Figure Lengend Snippet: Control and Paqr5 -silenced MBECs were cultured on large pore-size filter inserts until confluence. 4T1-tdTomato cells were placed in the top compartment at a density of 10 5 cells/cm 2 and left for 48 hours. A: Claudin-5 immunofluorescence was performed. One representative confocal micrograph is shown for both control and Paqr5 -silenced MBEC monolayers 48 hours after addition of the tumour cells. XY images show maximum intensity projection of z -stacks, while XZ and YZ views represent single optical slices along the light blue lines on the XY images. Arrows indicate bottom-to-top directions in the orthogonal sections. B: After removal of the cells from the top side of the filters, tumour cells that had migrated through the endothelial monolayer and the pores of the filter were counted. In the left panels, two representative epifluorescence images are shown for both conditions. Graph on the right represents average ± SD from N = 2 independent experiments, each performed in triplicates. Please note that the difference may be underestimated, as clusters of cells migrating through Paqr5 -silenced MBEC monolayers could not always be optically resolved into individual cells. **P ≤ 0.01 compared to control cells receiving the non-targeting siRNA (Student’s t -test).

Article Snippet: Cells from the top side of the filters were removed with a cotton swab and breast cancer cells migrated through the endothelial monolayer and the pores of the filter were imaged with an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a STEDYCON laser scanning confocal microscope (Abberior Instruments, Göttingen, Germany).

Techniques: Control, Cell Culture, Pore Size, Immunofluorescence

Journal: bioRxiv

Article Title: Within-host evolution of Klebsiella spp. from intestinal carriage to bacteremia

doi: 10.64898/2026.01.05.697662

Figure Lengend Snippet: (A.) Biofilm biomass produced after 4h of culture in M63B1 medium by the paired strains of Klebsiella isolated from rectal carriage (stool) or bacteriemia (blood), stained by crystal violet (Absorbance 570nm). Specific biofilm structure for concordant paired strains isolated from patient #5, CH1669 isolated from blood sample and CH1670 isolated from rectal swabbing alone (B.) and co-cultured (C.) Strains were followed in the BioFlux microfluidic system at 37°C under a shear force of 0.5 dyn/cm² using epifluorescence microscope (Axio observer 7, Zeiss) at a magnification of 20×. Images were acquired in real time at T = 5 h, 12 and 18h. CH1669, isolated from blood, competitiveness against CH1670 isolated from rectal swabbing (D.) ; K. pneumoniae CH1157 (E .) and E. coli TG1 (F.) . Each value is the mean ± SEM of at least three separate experiments. Statistical analysis: nonparametric Mann-Whitney test between paired strains: *p < 0.05, **p < 0.01.

Article Snippet: After a 15 min adhesion step at 37 °C without flow, biofilm growth was monitored at 37 °C under a shear force of 0.5 dyn/cm2 (63 μL/h) using an Axio Observer 7 inverted epifluorescence microscope (Zeiss, 20×).

Techniques: Produced, Isolation, Staining, Cell Culture, Shear, Microscopy, MANN-WHITNEY